50% off select Affinity Plus Probes (AffinityPlus50) | IDT

Dec 31, 2024  · This promotion is available to existing and new customers of IDT. This is a one-time promotion for 50% off select Affinity Plus Probes: LNA qPCR Probes (Iowa Black …


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Dec 31, 2024  · This promotion is available to existing and new customers of IDT. This is a one-time promotion for 50% off select Affinity Plus Probes: LNA qPCR Probes (Iowa Black …

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1 day ago  · 50% off Select Affinity Plus Probes . ... AffinityPlus50. Save Big. × Terms and conditions for AffinityPlus50 promo code. This promotion is available to existing and new …

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Oct 16, 2024  · Find Archer now at IDT! ... 50% off select Affinity Plus Probes (AffinityPlus50) Jan 2, 2025, 14:54 PM 50% off 2 xGen™ Library Prep Kits (50%2OrMore) ... 50% Off PrimeTime …

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Dec 31, 2024  · Terms and conditions for AffinityPlus50 promo code. ... This is a one-time promotion for 50% off select Affinity Plus Probes: LNA qPCR Probes (Iowa Black quenchers …

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FAQs about 50% off select Affinity Plus Probes (AffinityPlus50) | IDT Coupon?

What is an affinity plus qPCR probe?

An Affinity Plus qPCR Probe can be designed with several locked nucleic acid monomers resulting in a T m of >15°C, which greatly increases accuracy of allele determination in real-time PCR or other methods that use differential hybridization to distinguish polymorphisms. ...

Does a locked nucleic acid containing Affinity Plus qPCR probe generate identical genotyping calls?

NTC = no template control. The Figure 3 cluster plots from 4 genotyping assays demonstrate that locked nucleic acid containing Affinity Plus qPCR Probes generate identical genotyping calls as PrimeTime locked nucleic acid probes. ...

What are Affinity Plus oligonucleotides?

Affinity Plus Oligonucleotides provide identical annealing properties as other manufacturers' locked nucleic acid sequences (Figure 1). Oligonucleotides that have locked nucleic acids have enhanced stability, increased melting temperatures, and higher nuclease resistance, relative to unmodified oligonucleotides. Figure 1. ...

How do you enter Affinity Plus oligonucleotides?

To enter individual Affinity Plus nucleotides, insert a "+" before the base (+A, +C, +G, +T). Affinity Plus Oligonucleotides provide identical annealing properties as other manufacturers' locked nucleic acid sequences (Figure 1). ...

What is the relative binding affinity of Affinity Plus locked nucleic acid bases?

The relative binding affinity (T m) of Affinity Plus locked nucleic acid bases are Affinity Plus:Affinity Plus > Affinity Plus:DNA > DNA:DNA. Therefore, it is important to examine the probe sequence for self-dimer and hairpin formation and minimize designs that allow Affinity Plus:Affinity Plus pairing. ...

Where should Affinity Plus locked nucleic acid bases be placed?

Affinity Plus locked nucleic acid bases should be placed at the SNP site and on each of the adjacent bases. The SNP should be positioned in the center of the probe, if possible. Avoid placing Affinity Plus locked nucleic acid bases on the first or last bases of the probe sequence. ...

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